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PRODUCT INFORMATION

Serine Protease Inhibitor Cocktail I [Catalog # 28,010]

 

Lyophilized Solid (1 Vial)

Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and endanger all further downstream experiments. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material.

All cells contain a different mixture of enzymes but the following generalizations can be made: Serine proteases are widely distributed in most type of cells / Bacterial extracts typically contain serine and metalloproteases / Extracts from animal tissues contain mainly serine, cysteine and metalloproteases. Some also contain aspartic proteases / Plant extracts contain large amounts of serine and cysteine proteases.

 

Since cells contain different type of enzymes, our specially formulated cocktails of well selected, different inhibitors supplied in a ready-to-use form, will provide complete protection for your proteins of interest for subsequent experiments like Western blotting, reporter gene analysis, immunoprecipitations, eptitope tagging, specific protein activity assay, etc or during further purification steps.

 

Serine Protease Inhibitor Cocktail I

 

Product Description: White Lyophilized Solid. A cocktail of four protease inhibitors which has been optimized for the inhibition of a broad range of serine proteases.

Recommended Usage: Reconstitute each vial with 1 ml H2O to obtain a 100X concentrated stock solution. 1X stock solution contains the following components:

 

Products

Cat. No.

Mol. Wt.

 1X Concentration

Target Proteases

AEBSF, HCl

10,020

239.5

500 M

Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin and related thrombolytic enzymes.

Aprotinin

10,010

6512

420 nM

Inhibits chymotrypsin, coagulation factors, kallikrein, plasmin, tissue and leukocytic proteinases and trypsin.

Elastatinal

 

512.6

20 M

Inhibits elastase like serine proteases

GGACK

 

506.8

1 M

Inhibits urokinase and Factor Xa.

 

Storage / Stability: Freezer (-20oC). Hygroscopic. Following reconstitution, aliquot and freeze (-20oC). This product is stable for 3 years as supplied. Stock solutions are stable for up to 1 month at -20oC.

References: Proteolytic Enzymes- A Practical Approach (Beynon, P. J. & Bond, J. S. eds) 1994, pp. 241-249.

 

 

                Try  OTHER            Ready-to-Use

INHIBITOR COCKTAILS

 

 

Protease Inhibitor Cocktail I

Protease Inhibitor Cocktail II

Protease Inhibitor Cocktail III

Protease Inhibitor Cocktail IV

Protease Inhibitor Cocktail V, EDTA free

Protease Inhibitor Cocktail VI

Serine Protease Inhibitor Cocktail I

Phosphatase Inhibitor Cocktail I

Phosphatase Inhibitor Cocktail II

 

Cat. No.

25,100

25,110

25,120

25,130

25,140

25,150

28,010

25,160

25,170

AEBSF

10,020

X

X

X

X

X

X

X

 

 

Aprotinin

10,010

X

 

X

 

X

X

X

 

 

E-64

14,010

X

X

X

X

X

X

 

 

 

EDTA

 

X

X

 

 

 

X

 

 

 

Leupeptin

21,010

X

 

X

 

X

X

 

 

 

Bestatin

11,010

 

X

X

 

 

X

 

 

 

Pepstatin A

25,080

 

X

X

X

 

 

 

 

 

1,10-Phenanthroline

25,090

 

 

 

X

 

 

 

 

 

Elastatinal

 

 

 

 

 

 

 

X

 

 

GGACK

 

 

 

 

 

 

 

X

 

 

Bromotetramisole

11,020

 

 

 

 

 

 

 

X

 

Cantharidin

12,010

 

 

 

 

 

 

 

X

 

Microcystin LR

 

 

 

 

 

 

 

 

X

 

Imidazole

18,010

 

 

 

 

 

 

 

 

X

Sodium Fluoride

 

 

 

 

 

 

 

 

 

X

Sodium Molybdate

 

 

 

 

 

 

 

 

 

X

Sodium Orthovanadate

 

 

 

 

 

 

 

 

 

X

Sodium Tartrate

 

 

 

 

 

 

 

 

 

X

 

Related Products: Protein Solubilizers

Aldehyde & Peroxide Conc

< 0.1 mM

Conductivity

< 5 uMhos

Sterility

USP Grade

Concentration

10 0.5% (water for injection)

 

Highly purified, Sterile, 10% aqueous solution of Tween 80, Tween 20, Triton X-100, Triton X-114, NP-40 and Brij-35

 

Aseptically manufactured and filled in 10 X 5 ml amber prescored ampules

 

Sterile!  No more mold growth in your aqueous detergents

 

 

Products

Cat. No.

Pkg. Size

Protein Solubilizer X-114, Sterile 10% Triton X-114 Ampules

25,060

10 X 5ml

Protein Solubilizer 80, Sterile 10% Tween 80 Ampules

25,040

10 X 5ml

Protein Solubilizer X-100, Sterile 10% Triton X-100 Ampules

25,050

10 X 5ml

Protein Solubilizer 20, Sterile 10% Tween 20 Ampules

25,010

10 X 5ml

Protein Solubilizer 35, Sterile 10% Brij-35 Ampules

25,020

10 X 5ml

Protein Solubilizer 40, Sterile 10% NP-40 Ampules

25,030

10 X 5ml

Protein Solubilizer Set-1, Sterile 10% (Tween 20, Tween 80, Triton X-100, NP-40 & Brij-35) Ampules

25,070

5 Sample Set @     

2 X 5 ml each

 


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Last updated: 06/20/06.