Phosphatase Inhibitor Cocktail I [Catalog # 25,160]
Liquid. In 1 ml DMSO ( 1 ml )
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and endanger all further downstream experiments. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material.
All cells contain a different mixture of enzymes but the following generalizations can be made: Serine proteases are widely distributed in most type of cells / Bacterial extracts typically contain serine and metalloproteases / Extracts from animal tissues contain mainly serine, cysteine and metalloproteases. Some also contain aspartic proteases / Plant extracts contain large amounts of serine and cysteine proteases.
Since cells contain different type of enzymes, our specially formulated cocktails of well selected, different inhibitors supplied in a ready-to-use form, will provide complete protection for your proteins of interest for subsequent experiments like Western blotting, reporter gene analysis, immunoprecipitations, eptitope tagging, specific protein activity assay, etc… or during further purification steps.
Phosphatase Inhibitor Cocktail I
Product Description: Liquid. In 1 ml DMSO. A specially formulated cocktail of three phosphatase inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A.
Recommended Usage: Recommended for use with tissue and cell extracts including extracts containing detergents. Recommended usage is a 100-fold dilution. Each vial contains the following components:
Storage / Stability: Freezer (-20oC). This product is stable for 2 years as supplied.
References: Proteolytic Enzymes- A Practical Approach (Beynon, P. J. & Bond, J. S. eds) 1994, pp. 241-249.
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Highly purified, Sterile, 10% aqueous solution of Tween 80, Tween 20, Triton X-100, Triton X-114, NP-40 and Brij-35
Aseptically manufactured and filled in 10 X 5 ml amber prescored ampules
Sterile! No more mold growth in your aqueous detergents