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PRODUCT INFORMATION

Protein Solubilizer 35 [Catalog # 25,020]

Active Ingredient: Supplied as a highly purified,

Sterile, 10% aqueous solution of  Brij-35

 

Aseptically Manufactured and Filled

10 X 5 ml Ampoules

Chemically and Biologically Pure

Features                                                                                                         Benefits

Very low contaminating Aldehyde and Peroxide concentration

< 0.1 mM

 

To maximize yields of biologically active proteins

 

Extremely low conductivity

 < 5 ”mhos

 

 

Prevents problems associated with interfering salts and metal ions

 

10% solution in sterile water for injection

 

 

High purity and ease of dispensing of diluted solutions

 

Prescored 5 ml Amber Ampoules

 

 

Small package size with amber ampoules to maximize shelf life

 

Sterile, U.S.P Grade

 

 

To maintain biological purity

 

Aseptically manufactured and filled,

Large Scale GMP  capabilities

 

 

For Research use through therapeutic Production capabilities

 

Non-Ionic Detergents

 

Non-ionic detergents contain uncharged, hydrophilic head groups that consist of either polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions. Hence, they are considered non-denaturant and are widely used in the isolation of membrane proteins in their biologically active form.

 

The use and performance of a detergent is dependent on a number of general properties such as hydrophobicity, CMC, aggregation number, charge etc…(TABLE-1 : Properties of Non-Ionic Detergents).

 

Several investigators have reported problems associated with aldehyde and peroxide impurities in polyoxyethylene detergents 5,6,8. There has also been documented evidence of support of microbial growth and toxic metabolite production in non sterilized aqueous solutions of these detergents.

 

Our product line of Highly purified, Sterile, 10% aqueous solutions of detergents should offer a definite advantage to those working with sensitive membrane-bound proteins. Sterile! No more mold growth in your aqueous detergents.

 

 

TABLE -1: Properties of Non-Ionic Detergents

Product

Cat.No.

M.W.a

CMCb

(mM)

Agg.

No.

Avg.

Micellar

Wt.

HLB

No.

Cloud

Point

Protein Solubilizer 80

(10% Soln of Tween 80)

25,040

1310

0.012

58

76,000

15

-

Protein Solubilizer 20

(10% Soln of Tween 20)

25,010

1228

0.059

-

-

16.7

-

Protein Solubilizer X-100

(10% Soln of Triton X-100)

25,050

625

0.2-0.9

140

80,000

13

64șC

Protein Solubilizer X-114

(10% Soln of Triton X-114)

25,060

427

0.35

-

-

12.4

22șC

Protein Solubilizer 35

(10% Soln of Brij-35)

25,020

1198

0.09

40

48,000

16.9

-

Protein Solubilizer 40

(10% Soln of NP-40)

25,030

603

0.05-0.3

-

-

13.1

80șC

Protein Solubilizer Set-1

(5 Sample Set: 2X5 ml each)

25,070

-

-

-

-

-

-

a.        Average molecular weights for detergents composed of mixtures of chain lengths; b. Temperature: 20-25oC

 

References

  1. Banerjee, P., et al. (1993). Differential solubilization of membrane lipids by detergents. Arch. Biochem. Biophys. 305, 68.
  2. Neugebauer, J. M. (1990). Detergents: An overview. Methods. Enzymol. 182, 239.
  3. Furth, A. H., et al. (1984). Separating detergents from proteins. Methods. Enzymol. 104, 318.
  4. Hjelmeland, L. M., and Chrambach, A. (1984). Solubilization of functional membrane proteins. Methods. Enzymol. 104, 305.
  5. Ashani, Y. and Catravas, G. (1980). Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem. 109, 55-62.
  6. Chang, H. W. and Bock E. (1980). Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane proteins: sulfhydryl oxidizing contaminants and their elimination. Anal. Biochem. 104, 112-117.
  7. Helenius, A., McCaslin, D. R., Fries, E., and Tanford, C. (1979). Properties of detergents. Methods. Enzymol. 56, 734-749.
  8. Lever, M. (1977). Peroxides in detergents as interfering factors in biochemical analysis. Anal. Biochem. 83, 274.
  9. Slinde, E., and Flatmark, T. (1976). Effect of the hydrophile-lipophile balance of non-ionic detergents on the solubilization of biological membranes and their integral b-type cytochromes. Biochim. Biophys. Acta. 455, 796.
  10. Helenius, A. and Simons, K. (1975). Solubilization of membranes by detergents. Biochim. Biophys. Acta. 415, 29-79.

 

Brij is a registered trademark of ICI Surfactants.


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