Hygromycin B, Streptomyces sp.
Product: Hygromycin B
Description: An aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
Molecular Formula: C20H37N3O13
Molecular Weight: 527.5
CAS Number: 31282-04-9
Appearance: Lyophilized Solid.
Purity: ≥ 90% by HPLC. Potency: ≥1000 U/mg solid
Storage/Handling: Refrigerator (+4°C)
Shipping: Express Courier
Risk Statements: 26/27/28-37/38-41
Safety Statements: 22-26-36/37/39-45
References: Merck Index 13, 4878.
For laboratory use only