G 418 Sulfate
Product: G 418 Sulfate
Description: An aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
Molecular Formula: C20H40N4O10 · 2H2SO4
Molecular Weight: 692.7
CAS Number: 108321-42-2.
Appearance: White solid.
Purity: ≥ 98% by TLC; Potency: ≥730 µg/mg.
Storage/Handling: Room temp (+20°C)
Shipping: Express Courier
References: Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.; Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
For laboratory use only